|
KMID : 1140120080130010033
|
|
Cancer Prevention Research
2008 Volume.13 No. 1 p.33 ~ p.39
|
|
|
Induction of Caspase-independent Apoptosis by Methanol Extract of Gloiopeltis furcata in Human Hepatocarcinoma HepG2 Cells
|
|
Choi Woo-Young
Kim Cheng-Yun
Lee Won-Ho
Choi Yung-Hyun
|
|
|
Abstract
|
|
|
|
Previous results showed that the administration of seaweed powder or extract reduced the incidence rate of chemically induced tumorigenesis using in vivo animal model. Recently, we reported that the extracts of Gloiopeltis furcata, a kind of Korean edible seaweed, caused the cell growth inhibition of various human cancer cell lines, among them methanol extract exhibited a relatively strong antiproliferative activity. However, little is known about its precise effects and mechanisms of action in malignant cells. In the present study, we investigated the pathway of the induction of apoptotic cell death by methanol extract of G. furcata (MEGF) in human hepatocarcinoma HepG2 cells. It was found that MEGF could inhibit the cell viability and induce the apoptosis in a dose-dependent manner as measured by trypan blue counting, fluorescent microscope and flow cytometry analysis. Apoptosis induction of HepG2 cells by MEGF was associated with an down-regulation of anti-apoptotic Bcl-2 mRNA and protein expression, but other apoptosis-related genes including Fas/FasL system and IAP family members were not changed in MEGF-treated HepG2 cells. Moreover, MEGF treatment did not induce the proteolytic activation of caspase-3, -8 and -9, and degradation of poly(ADP-ribose) polymerase (PARP) protein suggesting that MEGF-induced apoptosis proceeds via a caspase-independent mechanism. Though further studies will be needed to identify the activity compounds that confer the anti-cancer activity of MEGF, the present findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of G. furcata. (Cancer Prev Res 13, 33-39, 2008)
|
|
|
KEYWORD
|
|
|
Gloiopeltis furcata, HepG2, Apoptosis, Caspase-independent
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|